職稱：教授，博導

學歷：博士

電話：0591-22866376

電子郵件：ljuan@fzu.edu.cn

研究方向：合成生物學，生物催化與酶分子工程，細胞工廠

教育工作經歷：

1988.9-1992.7：華東師范大學生物系，大學本科；

1998.9-2001.7：beat365在線體育官網生物化工專業，碩士研究生；

2003.9-2007.4：beat365在線體育官網分析化學專業，博士研究生；

1992.7-1995.4：福建農林大學；

1995.5-今：beat365在線體育官網beat365在線體育官網.

教學簡介：

beat365在線體育官網教學名師。近幾年承擔了中央財政支持地方高校發展專項資金項目《生物與食品學科研究生創新創業實踐基地建設》，beat365在線體育官網教改項目《微生物學實驗教學改革》、《微生物學雙語教學》、《細胞生物學網絡課程建設》、《細胞生物學精品課程建設》、《進一步完善我校本科生導師制的研究》、《微生物學系列課程教學團隊建設》、《生物工程類本科生創新創業實踐平臺建設》等10多項教改課題的研究，在高等教育出版社出版的《高校生命科學基礎課程報告論壇文集》、《beat365在線體育官網學報》（哲社版）、《高等教育研究》、《高教論壇》、《教育教學論壇》等刊物上發表教改論文10余篇。在2003、2004年的beat365在線體育官網第五屆、第六屆青年教師“最佳一節課”競賽活動中，分別獲得優秀獎和二等獎的好成績，并獲得福建省教學成果二等獎1項、beat365在線體育官網教學成果獎4項，7次獲得beat365在線體育官網教學優秀二等獎，2015年獲得beat365在線體育官網廈航獎教金。主講的《微生物學》和《細胞生物學》課程分別被評為福建省精品課程、beat365在線體育官網核心課程和beat365在線體育官網精品課程。

科研簡介：

福建省高等學校新世紀優秀人才支持計劃人選。主要從事合成生物學，生物催化與酶分子工程，工業微生物代謝工程改造。主持了國家863計劃子課題、國家海洋局海洋公益性行業科研專項子課題、福建省高校產學科技重大項目、福建省海洋高新產業發展專項、福建省經信委企業技術創新項目、福建省發改委生物產業專項、福建省自然科學基金重點項目、福建省科技廳重點項目以及產學研等30多項科研課題的研究工作；以第一作者或通訊作者發表SCI文章60多篇；授權國家發明專利18件。

社會兼職：

中國生物化學與分子生物學會酶學專業分會委員，福建省生物化學與分子生物學會副理事長；福建省微生物學會常務理事；福建省水產學會理事。

承擔的主要科研項目：

1、 白藜蘆醇生物合成技術開發，橫向項目，2024-2027；

2、 5-羥基色氨酸及褪黑素的生物合成技術開發，橫向項目，2024-2026；

3、 D-對甲砜基苯絲氨酸乙酯（D-乙酯）的產業化生產，橫向項目，2022-2026；

4、 甾醇類化合物酶催化技術開發，橫向項目，2022-2025；

5、 工業屬性高效生物催化劑的設計與開發，橫向項目，2019-2023；

6、 生物發酵功能飼料的開發，橫向項目，2019-2022；

7、 福建省科技創新重點項目“D-對甲砜基苯絲氨酸乙酯的生物催化新技術開發及產業化生產”，2022-2025；

8、 福建省基金重點項目“環己酮單加氧酶不對稱氧化奧美拉唑硫醚的分子機理研究”，2021-2024；

9、 福建省高校產學合作重大項目“高色價紅曲紅色素液態深層發酵關鍵技術開發”，2017-2020；

10、 福建省經信委企業技術創新項目“海洋藻類資源高值化利用技術創新平臺建設”，2015-2017；

11、 福建省海洋高新產業發展專項“魚罐頭加工廢棄液的高值化利用”，2015-2017；

12、 國家科技部863計劃子課題“新型糖酶創制與創新應用關鍵技術研究”，2013-2017；

13、 國家海洋局海洋公益性行業科研專項子課題“海洋生物酶資源庫及幾種海洋源性工業用酶關鍵生產技術開發”，2013-2016；

14、 福建省高校產學合作重大項目“利用現代微生物工程技術發酵生產超氧化物歧化酶”，2013-2016；

15、 福建省發改委生物產業項目“漆酶的產業化生產技術開發”，2011-2014。

以第一作者或通訊作者發表的主要論文：

1. Multienzymatic Cascade for Synthesis of Hydroxytyrosol via TwoStage Biocatalysis. Journal of Agricultural and Food Chemistry, 2024.

2. Single-cell enzymatic cascade synthesis of testolactone enabled by engineering of polycyclic ketone monooxygenase and multi-gene expression fine-tuning. International Journal of Biological Macromolecules, 2024.

3. Advances in Enzymatic Incorporation of Small Fluorine Modules. European Journal Organic Chemistry, 2024.

4. An artificial biocatalytic cascade for efficient synthesis of norepinephrine by combination of engineered L-threonine transaldolase with multi-enzyme expression fine-tuning. International Journal of Biological Macromolecules, 2024.

5. Development of whole cell biocatalytic system for asymmetric synthesis of esomeprazole with enhancing coenzyme biosynthesis pathway. Enzyme and Microbial Technology, 2024.

6. Efficiently manufacturing ectoine via metabolic engineering and protein engineering of L-2,4-diaminobutyrate transaminase. International Journal of Biological Macromolecules, 2024.

7. Immobilization of laccase on mesoporous metal organic frameworks for efcient crosscoupling of ethyl ferulate. World Journal of Microbiology and Biotechnology, 2024.

8. Rational design of short‐chain dehydrogenase/reductase for enantio‐complementary synthesis of chiral 1,2‐diols by successive hydroxymethylation and reduction of aldehydes. Biotechnology and Bioengineering, 2024.

9. Rational design on loop regions for precisely regulating flexibility of catalytic center to mitigate overoxidation of prazole sulfides by Baeyer-Villiger monooxygenase. Bioorganic Chemistry, 2024.

10. Batch and continuous flow asymmetric synthesis of anabolic-androgenic steroids via a single-cell biocatalytic Δ1 -dehydrogenation and C17β-carbonyl reduction cascade. Green Chemistry, 2023.

11. A chemoenzymatic process for preparation of highly purified dehydroepiandrosterone in high space-time yield. Bioorganic Chemistry, 2023.

12. A chemoenzymatic strategy for the efficient synthesis of amphenicol antibiotic chloramphenicol mediated by an engineered L-threonine transaldolase with high activity and stereoselectivity, Catalysis Science & Technology, 2023.

13. Efficient synthesis of 2’-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum. World Journal of Microbiology and Biotechnology, 2023.

14. Engineering of carbonyl reductase for asymmetric reduction of difficult-to-reduce ketone tetrahydrofuran-3-one. Molecular Catalysis, 2023.

15. Immobilization of laccase on magnetic nanoparticles for enhanced polymerization of phenols. Enzyme and Microbial Technology, 2023.

16. Improved Antimicrobial Activity of Bovine Lactoferrin Peptide (LFcinB) Based on Rational Design. The Protein Journal, 2023.

17. Multiplex metabolic pathway engineering of Monascus pilosus enhances lovastatin production. Applied Microbiology and Biotechnology, 2023.

18. Construction of an Efficient Non-natural Enzyme System for Preparation of Testosterone in High Space-Time Yield. ACS Sustainable Chemistry & Engineering, 2022.

19. Focused mutagenesis in non-catalytic cavity for improving catalytic efficiency of -ketosteroid -Δ1-dehydrogenase, Molecular Catalysis, 2022.

20. “Nonpolarity paving” in substrate tunnel of a Limnobacter sp. Phenylacetone monooxygenase for efficient single whole-cell synthesis of esomeprazole. Bioorganic Chemistry, 2022.

21. An Effective Chemo-Enzymatic method with An Evolved L-Threonine Aldolase for Preparing L-threo-4-Methylsulfonylphenylserine Ethyl Ester of High Optical Purity. Molecular Catalysis, 2022.

22. Engineered Cyclohexylamine Oxidase with Improved Activity and Stereoselectivity for Asymmetric Synthesis of a Bulky Dextromethorphan Precursor and Its Analogues. ChemCatChem, 2022.

23. Construction of efficient enzyme systems for preparing chiral ethyl 3-hydroxy- 3 -phenylpropionate. Enzyme and Microbial Technology, 2022.

24. Efficient biosynthesis of (2S, 3R)-4-methylsulfonylphenylserine by artificial self-assembly of enzyme complex combined with an intensified acetaldehyde elimination system. Bioorganic Chemistry, 2021.

25. Improving the Cβ Stereoselectivity of l-Threonine Aldolase for the Synthesis of l-threo-4-Methylsulfonylphenylserine by Modulating the Substrate-Binding Pocket To Control the Orientation of the Substrate Entrance. Chemistry—A European Journal, 2021.

26. Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability. Enzyme and Microbial Technology, 2021.

27. Single-Point Mutation Near Active Center Increases Substrate Affinity of Alginate Lyase AlgL-CD. Applied Biochemistry and Biotechnology, 2021.

28. Multi-enzyme cascade for improving ?-hydroxy-a-amino acids production by engineering L- threonine transaldolase and combining acetaldehyde elimination system. Bioresource Technology, 2020.

29. Asymmetric Synthesis of a Key Dextromethorphan Intermediate and Its Analogs Enabled by a New Cyclohexylamine Oxidase: Enzyme Discovery, Reaction Development, and Mechanistic Insight. Journal of Organic Chemistry, 2020.

30. A L-threonine aldolase for asymmetric synthesis of β-hydroxy-α-amino acids. Chemical Engineering Science, 2020.

31. Expression and characterization of a chitinase from Serratia marcescens. Protein Expression and Puri?cation, 2020.

32. Rational Design of Dehydrogenase/Reductases Based on Comparative Structural Analysis of Prereaction-State and Free-State Simulations for E?cient Asymmetric Reduction of Bulky Aryl Ketones. ACS Catalysis, 2019.

33. Characteristics of L-threonine transaldolase for asymmetric synthesis of β-hydroxy-α-amino acids. Catalysis Science & Technology, 2019.

34. Truncation of κ?carrageenase for higher κ?carrageenan oligosaccharides yield with improved enzymatic characteristics. International Journal of Biological Macromolecules, 2019.

35. Mutagenesis on the surface of a β-agarase from Vibrio sp. ZC-1 increased its thermo-stability. Enzyme and Microbial Technology, 2019.

36. Inhibitory mechanism of Penicillin V on mushroom tyrosinase. Molecular Biology Reports, 2019.

37. Prediction of zinc-binding sites using multiple sequence profiles and machine learning methods. Molecular Omics, 2019.

38. Preparation of bioactive neoagaroligosaccharides through hydrolysis of Gracilaria lemaneiformis agar: A comparative study. Food Chemistry, 2018.

39. A Novel Multi-domain High Molecular, Salt-Stable Alkaline Xylanase from Alkalibacterium sp. SL3. Frontiers in Microbiology, 2017.

40. Inactivation kinetics and conformation change of Hypocrea orientalis b-glucosidase with guanidine hydrochloride. Journal of Bioscience and Bioengineering, 2017.

41. Degradation of tetracycline by immobilized laccase and the proposed transformation pathway. Journal of Hazardous Materials, 2017.

42. Immobilized Cerrena sp. Laccase preparation, thermal inactivation, and operational stability in malachite green decolorization. Scientific Reports, 2017.

43. Laccases Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation. Frontiers in Microbiology, 2017.

44. A novel cold-adapted and highly salt-tolerant esterase from Alkalibacterium sp. SL3 from the sediment of a soda lake. Scientific Reports, 2016.

45. Cross-linked enzyme aggregates of Cerrena laccase: Preparation, enhanced NaCl tolerance and decolorization of Remazol Brilliant Blue Reactive. Journal of the Taiwan Institute of Chemical Engineers, 2016.

46. Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with fungal laccase. Analytical Biochemistry, 2016.

47. Laccase Production and Differential Transcription of Laccase Genes in Cerrena sp.in Response to Metal Ions, Aromatic Compounds, and Nutrients. Frontiers in Microbiology, 2016.

48. Optimal parameters for laccase-mediated destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels. Data in Brief, 2016.

49. A neural network learning approach for improving the prediction of residue depth based on sequencederived features. RSC Advances, 2016.

50. Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis. Molecules, 2016.

51. Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris. Biotechnology and Applied Biochemistry, 2016.

52. The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Biochem Biophys Res Commun, 2016.

53. Laccase-Catalyzed Decolorization of Malachite Green: Performance Optimization and Degradation Mechanism. PLOS ONE, 2015.

54. Molecular characterization of a thermophilic and salt and alkaline-tolerant xylanase from Planococcus sp. SL4, a strain isolated from the sediment of a soda lake. Journal of Microbiology and Biotechnology（JMB）, 2015.

55. Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris, Biotechnology and Applied Biochemistry, 2015.

56. A novel laccase from basidiomycete Cerrena sp.: Cloning, eterologousexpression, and characterization. International Journal of Biological Macromolecules, 2015.

57. Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability. PLOS ONE, 2014.

58. High Phylogenetic Diversity of Glycosyl Hydrolase Family 10 and 11 Xylanases in the Sediment of Lake Dabusu in China. PLOS ONE, 2014.

59. Antioxidant activities of kombucha prepared from three different substrates and changes in content of probiotics during storage. Food Science and Technology, 2014.

獲獎情況：

教學：分別獲得beat365在線體育官網第五屆、第六屆青年教師“最佳一節課”競賽優秀獎和二等獎，并獲得福建省教學成果二等獎1項，beat365在線體育官網教學成果獎4項，7次獲得校教學優秀二等獎，2015年獲得beat365在線體育官網廈航獎教金。

科研：獲省科技進步三等獎1項，2007年入選福建省高等學校新世紀優秀人才支持計劃。